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KMID : 0644120140270040001
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Journal of Oriental Gynecology
2014 Volume.27 No. 4 p.1 ~ p.14
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Antimetastatic Effects of Jipae-san by Inflammation Control and Activation of Innate Immune System
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Heo Su-Jeong
Hwang Deok-Sang
Lee Jin-Moo
Lee Chang-Hoon
Lee Kyung-Sub
Jang Jun-Bock
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Abstract
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Objectives: This study was designed to investigate the anti-tumor metastasis by anti-inflammatory and innate immunomodulating effects of extracts of Jipae-san on cancer cells.
Methods: Antimetastatic experiments were conducted in vivo mouse model by using 4T1 mouse mammary carcinoma cells. Cell viability of Jipae-san was tested with 4T1 mouse mammary carcinoma cells, colon 26-M3.1 carcinoma cells and macrophage. In addition expression of TNF-α and NO induced by LPS was measured after treating with Jipae-san. To observe innate immunomodulating effects of Jipae-san on macrophage, we measured TNF-α, IL-12, IL-6 and MCP-1, respectively. Cell cytotoxicity was tested with the macrophage stimulated with Jipae-san and we evaluated the activation of TNF-α and NO. And the effect of Jipae-san on metastasis was measured without NK-cell using GM1 serum.
Results: Intravenous inoculation of Jipae-san significantly inhibited metastasis of 4T1 mouse mammary carcinoma cells. In an in vitro cytotoxicity analysis, cell growth are closer to 100% less than 1,000 μg/ml concentration. The expression of TNF-α and NO induced by LPS after treating Jipae-san was down regulated in dose-dependent manner. Level of cytokines such as TNF-α, IL-12, IL-6 and MCP-1 of Jipae-san group were up regulated in compared to the control group. The macrophage stimulated with Jipae-san significantly inhibits the cancer cell at ratio of 10:1, 20:1. The activation of NO was significantly up regualted in a group of 5:1, 10:1, 20:1. The depletion of NK-cells by anti-asialo GM1 serum partly abolished the inhibitory effect of Jipae-san on tumor metastasis.
Conclusions: Jipae-san appears to have considerable activity on the anti-metastasis by inflammation control and activation of innate immune system.
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KEYWORD
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Jipae-san, Cancer, Inflammation control, Innate immune system, Anti-metastasis, Complementary therapies
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